CSHL Single Cell Biology Core
Key Services:
10X Genomics Single Cell Flex (Fixed RNA Profiling)
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Up to 128,000 cells per lane across up to 16 samples
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Probe-based RNA profiling
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Batch together samples collected and fixed at different times or from FFPE tissue blocks.
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Lowest cost per sample
10X Genomics Single Cell 3’ Gene Expression
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2,000-20,000 cells per lane
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Oligo-d(T) primed, 3'-biased digital gene expression
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Best option for pure expression profiling
Add-ons:
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Cell Hashing / CellPlex multiplexing
10X Genomics Single Cell 5' Gene Expression
Enables Single Cell Immune Profiling:
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Paired TCR alpha- and beta chain V(DJ) sequencing
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Paired BCR heavy and light chain V(DJ) sequencing
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Combined Immune Profiling 5’-Gene Expression
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BEAM: (Bar-Enabled) Antigen Mapping
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Identify B- or T-cell clones binding to a labeled antigen
10X Genomics Single Cell ATAC-seq
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Genome-wide Chromatin Accessibility in single cells
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2,000-8,000 cells per lane
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Promoter / enhancer accessibility
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Motif enrichment / discovery
10X Genomics Multiome ATAC + Gene Expression
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2,000-8,000 cells per lane
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Paired 3’ nuclear gene expression and chromatin accessibility from single cells
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Analyze relationships between regulatory motifs and gene expression in multiple lineages simultaneously
Frozen Nuc-Seq
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Fresh or flash-frozen tissue
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On-site FACS sorting & direct-to-10X workflow
