CSHL Single Cell Biology Core
BOOK YOUR SEAT
10x Flex scRNA-seq $627/sample
No Frills Single Cell Sequencing. Fixed samples, probe based workflow. Benefit from the economies of scale by flying budget with your colleagues. Once the (p)lane fills with 12 or more samples, we take off!
Send us your fixed tissue
Fix your tissue using the 10x Flex Sample Prep Kit (core provided)
Dissociate, Capture, Sequence
We'll dissociate (if necessary), Capture 10,000-20,000 cells
Sequence to 10,000 reads/cell
Primary Analysis
We'll provide basic primary analysis, cell typing and sample aggregation
What to Pack?
Submit fixed pieces of tissue according to the 10x guide. Stop at page 3 and freeze the samples. Alternatively submit 300,000 fixed cells according to this protocol.
10x Flex Apex is a probed-based, "whole-ish transcriptome" gene expression assay for Human, Mouse and Rat samples. Details about the probe sets can be found here.
Find your Gate
Once you've requested Budget Flex in ilab, drop frozen samples off at Woodbury (main campus pickup coming soon). Samples will be processed using the 10x Flex Apex Kit once we receive 12 samples to run together.
What's in your ticket?
Ready to Upgrade?
Sometimes economy just won't cut it. Check out our full service suite of single cell products:
10X Genomics Single Cell 3' Gene Expression
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2,000-20,000 cells
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Oligo-d(T) primed, 3'-biased digital gene expression
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Non-biased - capture transgenes, lncRNAs, non human/mouse/rat species
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Generates full length cDNA- long read compatible, capture SNPS, isoforms and more
10x Flex Apex
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Individualized attention
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Sample prep optimization including sorting, custom fixation/ dissociation conditions
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Spike in custom probes
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Don't wait on anyone else
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Customize per sample input relative to each other
Single Cell 5' Gene Expression and Immune Profiling
Enables Single Cell Immune Profiling:
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Paired TCR alpha- and beta chain V(DJ) sequencing
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Paired BCR heavy and light chain V(DJ) sequencing
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Combined Immune Profiling 5’-Gene Expression