Equipment

Sony SH800 Sorter

FACS sorter, capable of 6-color indexed sorting and collection in tubes, 96-well, or 384-well plates.
Fluor picking chart

10X Genomics Chromium Controller

Uses microfluidic chips to capture cells for droplet based single cell genomics assays from 10x Genomics. Captures up to 10,000 cells per sample on up to 8 samples per 20 minute run.

Countess II FL Automated Cell Counter

Countess II FL Automated Cell Counter
Automated Cell Counter compatible with brightfield based viability dyes (eg. Trypan Blue) and fluorescent dyes using the Texas Red, GFP and DAPI light cubes

NextSeq 2000

The CSHL NGS Core offers Illumina Sequencing on the NextSeq 2000 platform with P2 flowcells (~500 Million reads) or P3 flowcells (~1 Billion reads)

Illumina NextSeq 500 and MiSeq is also available.

Long Read Sequencing

The CSHL NGS Core offers long read sequencing with the Oxford Nanopore PromethION and MinION and PacBio Sequel II sequencing on whole transcriptome libraries or target enriched libraries.

Agilent Bioanalyzer 2100

cDNA and final libraries are evaluated by Agilent 2100 Bioanalyzer before sequencing is performed to ensure samples are of high quality.