CSHL Single Cell Biology Core
Single Cell Biology @
Cold Spring Harbor's Single Cell Biology Core Facility brings cutting-edge single cell technologies to collaborators both inside and outside of the Laboratory. We currently specialize in single cell transcriptomics, and offer assistance in a variety of gene expression workflows based around the 10X Genomics platform.
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External Links
Key Services:

Plate Based 3’ Well Barcoded Low Input RNA-seq:
​Up to 96 Samples per library of well barcoded RNAseq
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You provide:
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15 ul of purified RNA at 25 ng/ul or more per sample
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Or 96 well Tissue Culture plate of cells frozen in lysis buffer
We:​
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Perform Well Barcoded RT reaction and prepare one library per plate
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Sequence using the Illumina NextSeq platform
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Perform primary analysis, mapping to a genome of your choice and return, FASTQs, bam files and a per sample gene counts matrix
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Nuc-Seq
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Fresh or flash-frozen tissue
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On-site FACS sorting & direct-to-10X workflow

Add on Services
Antibody-based proteogenomics
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Custom amplicon enrichment
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Core-assisted design of PCR probes