CSHL Single Cell Biology Core
Single Cell Biology @ CSHL
Cold Spring Harbor's Single Cell Biology Core Facility brings cutting-edge single cell technologies to collaborators both inside and outside of the Laboratory. We currently specialize in single cell transcriptomics, and offer assistance in a variety of gene expression workflows based around the 10X Genomics platform.
Main Services:
10X Genomics Single Cell 3’ Gene Expression
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2,000-8,000 cells per lane
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Oligo-d(T) primed, 3'-biased digital gene expression
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Best option for pure expression profiling
10X Genomics Single Cell 5' Gene Expression
Enables Single Cell Immune Profiling:
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Paired TCR alpha- and beta chain V(DJ) sequencing
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Paired BCR heavy and light chain V(DJ) sequencing
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Combined Immune Profiling 5’-Gene Expression
10X Genomics Single Cell ATAC-seq
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Genome-wide Chromatin Accessibility in single cells
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2,000-8,000 cells per lane
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Promoter / enhancer accessibility
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Motif enrichment / discovery
10X Genomics Multiome ATAC + Gene Expression
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2,000-8,000 cells per lane
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Paired 3’ gene expression and chromatin accessibility from single cells
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Analyze relationships between regulatory motifs and gene expression in multiple lineages simultaneously
10X Genomics Visium Spatial Transciptomics
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Spatially resolved transcriptome data
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Analyze tissue sections up to 6.5 x 6.5 mm
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Resolution of 1-10 cells
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Spot width 55uM, center-to-center distance 100uM
Plate Based 3’ Well Barcoded Low Input RNA-seq:
Up to 96 Samples per library of well barcoded RNAseq
You provide:
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15 ul of purified RNA at 25 ng/ul or more per sample
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Or 96 well Tissue Culture plate of cells frozen in lysis buffer
We:
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Perform Well Barcoded RT reaction and prepare one library per plate
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Sequence using the Illumina NextSeq platform
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Perform primary analysis, mapping to a genome of your choice and return, FASTQs, bam files and a per sample gene counts matrix
Nuc-Seq
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Fresh or flash-frozen tissue
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On-site FACS sorting & direct-to-10X workflow
Add on Services
Antibody-based proteogenomics
Custom amplicon enrichment
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Core-assisted design of PCR probes